《植物生理学报》 2009, 45(5): 444-448

东方山羊豆液泡膜Na⁺/H⁺ 逆向转运蛋白基因的克隆与分析

李鑫^1,2, 王赞², 王学敏², 高洪文^1,2,*, 陈小芳², 董洁², 徐博²

1 兰州大学草地农业科技学院, 兰州730020; 2 中国农业科学院北京畜牧兽医研究所, 北京100193

通信作者：高洪文；E-mail: gaohongwen@263.net；Tel: 010-62894560

摘　要：

采用 RT-PCR 及 RACE 的方法从东方山羊豆中克隆了一种新的 Na+/H+ 逆向转运蛋白基因。该基因全长 2 280 bp, 开 放阅读框为 1 626 bp, 编码 542 个氨基酸残基。预测该蛋白分子量为 60.0 kDa, 可能有 10 个跨膜区域。RT-PCR 检测结果表 明, 在 NaCl 胁迫条件下东方山羊豆 GoNHX1 基因可在根、茎、叶中诱导表达, 在叶中的表达量随着处理时间的延长而逐 渐增加, 到 24 h 表达量下降。

关键词：东方山羊豆; Na+/H+逆向转运蛋白; GoNHX1; RACE

收稿：2009-01-04   修定：2009-03-16

资助：国家“973” 项目(2007CB108905)和“ 十一五” 科技支撑项目(2007BAD56B02; 2008BADB3B01)。

Cloning and Analysis of a Vacuolar Na⁺/H⁺ Antiporter Gene in Galega orientalis

LI Xin^1,2, WANG Zan², WANG Xue-Min², GAO Hong-Wen^1,2,*, CHEN Xiao-Fang², DONG Jie2, XU Bo²

¹College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China; ²Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Corresponding author: GAO Hong-Wen; E-mail: gaohongwen@263.net; Tel: 010-62894560

Abstract:

The full length cDNA of a new vacuolar Na⁺/H⁺ antiporter gene (GoNHX1) in Galega orientalis was cloned by RT-PCR and RACE. The data showed that the full length of GoNHX1 gene was 2 280 bp, the open reading frame of GoNHX1 was 1 626 bp and encoded a putative protein of 542 amino acid residues. The analysis showed that the putative protein was 60.0 kDa and had 10 potential transmembrane domains. RT-PCR results revealed that GoNHX1 expression could be induced in the roots, stems and leaves by NaCl. The expression level of GoNHX1 in leaves increased with the increasing of treatment time, but decreased after 24 h with the treatment of NaCl.

Key words: Galega orientalis; Na⁺/H⁺ antiporter; GoNHX1; RACE